Entry 22 of 630 from Query 38   * Names only * * Complete entries * CABRI_Cell_Lines_View CABRI_Std_View

Entry Information           Entry from: DSMZ_MUTZ     Entry Options           Link to related information: Save entry: View:

commonly 2-3 days) CABRI:DSMZ_MUTZ Accession_number ACC 23 Cell_line_name 7-TD-1 Brief_description confirmed as mouse with IEF of MDH, NP, PEP B murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6 Description mouse B cell hybridoma murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6 confirmed as mouse with IEF of MDH, NP, PEP B Depositor Dr. E. Schmitt, Institute of Immunology, University of Mainz, Mainz, Germany Morphology round cells, growing single or clustered in suspension Culture_conditions 90% RPMI 1640 + 5% FBS + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine maintain at 0.1-0.3 x 106 cells/ml, optimal split ratio (daily) of 1:3, maximal split ratio of 1:5; for an IL-6 bioassay, we recommend to initially use 1.25-2.5 x 104 cells/ml and 100 microl in a 96-well-plate (it is advisable to determine the optimal cell density in various growth curves) at 37C with 7.5% CO2 maximum density of 0.5-1.0 x 106 cells/ml Viruses ELISA: reverse transcriptase positive; PCR: SMRV - Properties bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell dilutions beginning with cell densities of 2.5 x 104 cells/ml; duration of assay is Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 105 cells/ampoule Doubling_time ca. 24-25 hours Mycoplasma contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, Cytogenetics murine karyotype Bibliographic_reference Van Snick et al., Proc Natl Acad Sci USA, 83: 45 [PMID: 2948184 ]

SRS Release 7.1.3    Copyright © 1997-2003 LION bioscience AG. All Rights Reserved. Terms of Use Feedback